Hyperbranched polymers and polyplexes and DNA or RNA delivery systems including the same

A hyperbranched polyplex, comprising:

• a plurality of self-assembled hyperbranched polymers, each hyperbranched polymer including:
• a hyperbranched, hydrophobic molecular core;
• respective low molecular weight polyethyleneimine chains attached to two or more branches of the hyperbranched, hydrophobic molecular core;
• and
• respective polyethylene glycol chains attached to two or more other branches of the hyperbranched, hydrophobic molecular core;
• wherein the hyperbranched polyplex is a 3-layer nanosphere or nanoparticle in which:
• a first plurality of the polyethylene glycol chains forms a hydrophilic inner section of the polyplex;
• a second plurality of the polyethylene glycol chains forms a hydrophilic outer section of the polyplex;
• the hyperbranched, hydrophobic molecular cores and the low molecular weight polyethyleneimine chains form a central section positioned between the hydrophilic inner and outer sections;
• and
• the central section further includes DNA or RNA biomolecules complexed with the low molecular weight polyethyleneimine chains.