Compositions and methods for promoting immune responses to human immunodeficiency virus
AssigneesEmory University, Indian Institute of Science
InventorsRama Rao Amara, Andrew Jones, Raghavan Varadarajan
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Abstract
This disclosure relates to methods of promoting immune responses against HIV and compositions related thereto. In certain embodiments, this disclosure relates to methods of vaccinating for HIV comprising administering to the subject a priming composition followed by a boosting composition. In certain embodiments, the priming composition comprises a recombinant virus such as recombinant MVA that encodes an Env protein of HIV or segment thereof. In certain embodiments, the boosting composition comprises a trimeric cyclically permuted gp120 chimeric protein reported herein or DNA encoding the same.
Claims6
A method of vaccinating comprising:
- i) administering to a subject a nucleic acid and/or recombinant virus that encodes an HIV Env protein or segment thereof under conditions such that virus-like particles with surface gp120 proteins are formed in the subject;
- ii) administering to the subject an effective amount of a chimeric protein comprising an amino acid sequence selected from SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and SEQ ID NO:
- 5, and;
- wherein administering is to the buccal cavity propelled through the skin or soft tissue lining of the mouth with pressure.
A composition comprising a chimeric protein comprising an amino acid sequence
- (SEQ ID NO:
- 2)
- EEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAILENTVVASSEG
- TMMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNS
- NKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNG
- TGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKN
- ILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCN
- VSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGG
- EFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMAAA
- WQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGD
- MRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVGREKRGSAGSAGSSRS
- AGSAGSAGSEIHLENVTEEFNMWKNNMVEQMFITDIISLWDQSLKPCV
- KLTPLCVTLQCTNVTNNITDDAAA.
A composition comprising a chimeric protein comprising an amino acid sequence
- (SEQ ID NO:
- 3)
- EEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAILENTVVASSVN
- VTNGEEIKNCSFNATTEIRDKKQKVYALFYRLDIVPLEEERKGNSSKY
- RLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPC
- NNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVH
- LNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAYCNIKKD
- DWIRTLQRVGKKLAEHFPRRIINFTSPAGGDLEITTHSFNCRGEFFYC
- NTSSLFNSTYNPNDTNSNSSSSNSSLDITIPCRIKQIINMWQEVGRAM
- YAPPIEGNITCKSNITGLLLVRDGGVESNETEIFRPGGGDMRNNWRSE
- LYKYKVVEIKPLGIAPTAAKRRVVEREKRGSAGSAGSSRSAGSAGSAG
- SEMVLENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTL
- ECRQVNTTNATSAAA.
A composition comprising a chimeric protein comprising an amino acid sequence
- (SEQ ID NO:
- 4)
- EEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAILENTVVASSVN
- VTNGEVMKNCSFNATTELKDKKHKVHALFYKLDVVPLNGNSSSSGEYR
- LINCNTSAITQACPKVSFDPIPLHYCAPAGFAILKCNNKTFNGTGPCR
- NVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHL
- NESVNINCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAHCNINESK
- WNNTLQKVGEELAKHFPSKTIKFEPSSGGDLEITTHSFNCRGEFFYCN
- TSDLFNGTYRNGTYNHTGRSSNGTITLQCKIKQIINMWQEVGRAIYAP
- PIEGEITCNSNITGLLLLRDGGQSNETNDTETFRPGGGDMRDNWRSEL
- YKYKVVEIKPLGVAPTEAKRRVVEREKRGSAGSAGSSRSAGSAGSAGS
- EMVLANVTENFNMWKNDMVEQMHEDIISLWDESLKPCVKLTPLCVTLN
- CTNVKGNESDTAAA.
A composition comprising a chimeric protein comprising an amino acid sequence
- (SEQ ID NO:
- 5)
- EEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAILENTVVASSVN
- VTNGEEIKNCSFNTTTEIRDKKQKEYALFYRLDIVPLNEERKGNSSEY
- RLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPC
- NNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVH
- LNESVEINCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISES
- KWNKTLQRVSKKLKEHFPNKTIKFAPSSGGDLEITTHSFNCRGEFFYC
- NTSKLFNSTYNNNTSNSTITLPCRIKQIINMWQEVGRAMYAPPIAGNI
- TCKSNITGLLLTRDGGNNNNTTETFRPGGGDMRDNWRSELYKYKVVEI
- KPLGIAPTKAKRRVVEREKRGSAGSAGSSRSAGSAGSAGSEMVLENVT
- ENFNMWKNDMVDQMHEDTTSLWDQSLKPCVKLTPLCVTLNCTNVNVTN
- ATSAAA.
Dependencies are inferred from the claim text.