Degradation pathway for pentose and hexose sugars
AssigneeBraskem S.A.
InventorsDaniel Johannes Koch, Lucas Pederson Parizzi, Felipe Galzerani, Jean Marie Francois, Sophie Lajus
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Abstract
The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) or glycolic acid (GA), or MEG and one or more co-product, from one or more pentose and/or hexose sugars. Also provided are methods of producing MEG (or GA), or MEG (or GA) and one or more co-product, from one or more pentose and/or hexose sugars using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA), or MEG and one or more co-product.
Claims17
A recombinant microorganism overexpressing an enzyme having D-ribose-5-phosphate aldolase (DERA) activity,
- wherein said microorganism produces one or more pentose-phosphate intermediates from one or more pentose and/or hexose sugars, wherein the one or more pentose phosphate intermediates is selected from D-ribose-5-phosphate, D-ribulose-5-phosphate or D-xylulose-5-phosphate,
- wherein said enzyme converts the one or more pentose phosphate intermediates to glyceraldehyde-3-phosphate (G3P) and glycolaldehyde, and wherein said enzyme comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 256 or SEQ ID NO:
- 297, and
- wherein said microorganism produces one or more products from the G3P and the glycolaldehyde, wherein the one or more products is selected from monoethylene glycol (MEG) and glycolic acid (GA).
Dependencies are inferred from the claim text.