Terpene synthase producing patchoulol and elemol, and preferably also pogostol
AssigneeIsobionics B.V.
InventorsMartinus Julius Beekwilder, Adèle Margaretha Maria Liduina Van Houwelingen, Hendrik Jan Bosch, Georg Friedrich Lentzen, Elena Melillo
Life of this patent
Estimating the term…
Abstract
The invention is directed to a patchoulol synthase, to a nucleic acid encoding said patchoulol synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing patchoulol and elemol, and preferably also pogostol, and to a method of preparing a patchoulol synthase.
Claims20
A patchoulol synthase having an amino acid sequence which has a sequence identity of at least 95% but less than 100% with SEQ ID NO:
- 4.
A nucleic acid comprising a nucleic acid sequence as shown in SEQ ID NO:
- 5, or a nucleic acid sequence having a sequence identity of at least 95%, with the sequence shown in SEQ ID NO:
- 5, or a complementary sequence of any of these sequences.
A patchoulol synthase comprising an artificial fusion protein comprising a peptide or protein tag sequence and an amino acid sequence as shown in SEQ ID NO:
- 4 or a functional homologue thereof, said homologue being a patchoulol synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 4.
A method for preparing patchoulol and elemol, and pogostol, comprising converting a farnesyl diphosphate to patchoulol and elemol, and pogostol in the presence of a patchoulol synthase comprising an amino acid sequence as shown in SEQ ID NO:
- 4 or a functional homologue thereof, said homologue being a patchoulol synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 4, wherein the method prepares elemol, patchoulol and pogostol, and wherein the ratio of pogostol to patchoulol is higher than 1:5.
Dependencies are inferred from the claim text.