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US 11,584,922 B2Granted
Protease and binding polypeptide for O-glycoproteins
AssigneeGenovis AB
InventorsFredrik Leo, Rolf Lood, Stephan Bjork, Malin Mejare, Fredrik Olsson
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Abstract
The present invention relates to a novel endoprotease, mutants thereof having binding but lacking or having reduced hydrolyzing activity, and use in methods of studying and isolating O-linked glycoproteins.
Claims21
A method of hydrolysing an O-glycoprotein, wherein the method comprises contacting a sample comprising the O-glycoprotein with a polypeptide having endoprotease activity specific for O-glycosylated proteins which comprises:
- (a) the amino acid sequence of SEQ ID NO:
- 1;
- or
- (b) an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1 and comprises a motif comprising the sequence HEIGH (SEQ ID NO:
- 42) or HELGH (SEQ ID NO:
- 41).
4 more elements
A method for assessing the glycosylation status of a protein, comprising:
- contacting a sample comprising the protein with a polypeptide having endoprotease activity specific for O-glycosylated proteins which comprises:
- (a) the amino acid sequence of SEQ ID NO:
- 1;
- or
- (b) an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1 and comprises a motif comprising the sequence HEIGH (SEQ ID NO:
- 42) or HELGH (SEQ ID NO:
- 41);
- and
- detecting and/or analysing the products produced.
7 more elements
Dependencies are inferred from the claim text.