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US 11,584,783 B2Granted
Processes for producing a fermentation product
AssigneeNovozymes A/S
InventorsMarc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Abstract
The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.
Claims10
A process of producing a fermentation product, comprising (a) liquefying a starch-containing material with an alpha-amylase;
- optionally presaccharifying the liquefied material before step (b);
- (b) saccharifying the liquefied material;
- (c) fermenting using a fermentation microorganism;
- wherein
- i) a glucoamylase;
- and
- ii) a polypeptide having trehalase activity selected from the group consisting of a polypeptide having at least 93% sequence identity to the mature polypeptide of SEQ ID NO:
- 21 and a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 20 or the cDNA sequence thereof;
- and
- iii) optionally a cellulolytic enzyme composition, a protease, or both a cellulolytic enzyme composition and a protease are present or added during
- the saccharification step (b);
- the fermentation step (c);
- or
- the optionally presaccharification step before step (b).
12 more elements
A process of producing fermentation products from starch-containing material comprising:
- (i) saccharifying a starch-containing material at a temperature below the initial gelatinization temperature;
- and
- (ii) fermenting using a fermentation microorganism;
- wherein saccharification, fermentation, or saccharification and fermentation is done in the presence of the following enzymes:
- a. glucoamylase;
- b. alpha-amylase;
- and
- c. a polypeptide having trehalase activity selected from the group consisting of a polypeptide having at least 93% sequence identity to the mature polypeptide of SEQ ID NO:
- 21 and a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 20 or the cDNA sequence thereof;
- and
- d. optionally a protease, a cellulolytic enzyme composition, or a protease and a cellulolytic composition.
9 more elements
Dependencies are inferred from the claim text.