Pseudomonas strains and their metabolites to control plant diseases
AssigneesT3 Bioscience, LLC, UWM Research Foundation, Inc.
InventorsChing-Hong Yang, Xiangyang Liu
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Abstract
The present disclosure concerns methods of using novel bacterial strains of 0617-T307, 0917-T305, 0917-T306, 0917-T307, 0118-T319, 0318-T327, and 0418-T328, the cell broth and novel metabolites produced from the bacterial strains, that can inhibit the growth of a variety of microbial species for a variety of crops. The methods include use of novel, potent antimicrobial metabolites produced from the strains corresponding to compounds having Formulas (I), (II), and (III):
Claims27
A method of controlling a bacterial crop disease, comprising the steps of
- (i) producing an agricultural composition comprising:
- (a) a bacterial fermentate produced from a Pseudomonas bacteria selected from the group consisting of Pseudomonas soli 0617-T307 (Accession No. PTA-126796), Pseudomonas soli 0917-T305 (Accession No. PTA-126797), Pseudomonas soli 0917-T306 (Accession No. PTA-126798), Pseudomonas soli 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802);
- or a protective supernatant derived therefrom;
- or
- (b) an agriculture composition comprising at least one of Formula (I) or Formula (II):
- and
- (ii) applying said agricultural composition a crop to inhibit the growth of a pathogenic microorganism.
The method of 1, wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
A method of controlling a bacterial crop disease, comprising:
- applying an agricultural composition comprising between about 1.0×105 and 1.0×109 cfu per mL Pseudomonas bacteria to a crop to inhibit the growth of a pathogenic microorganism,
- wherein the Pseudomonas bacteria is selected from the group consisting of Pseudomonas soli and Pseudomonas mosselii.
A method of a controlling bacterial crop disease, comprising:
- (i) producing an agricultural composition comprising a bacterial fermentate from Pseudomonas bacteria selected from the group consisting of Pseudomonas soli 0617-T307 (Accession No. PTA-126796), Pseudomonas soli 0917-T305 (Accession No. PTA-126797), Pseudomonas soli 0917-T306 (Accession No. PTA-126798), Pseudomonas soli 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802), and
- (ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism.
The method of 11, wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
A method of controlling a bacterial crop disease, comprising the steps of
- (i) producing an agricultural composition comprising at least one of Formula (I) or Formula (II):
- and
- (ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism.
The method of 20, wherein the bacterial crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
The method of 24, wherein the bacterial crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.
Dependencies are inferred from the claim text.