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AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College, The University of Tokyo
InventorsFeng Zhang, Winston Yan, Osamu Nureki, Kaijie Zheng, Le Cong, Hiroshi Nishimasu, Fei Ran, Yinqing Li
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The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.
An engineered SaCas9 protein comprising at least one modification compared to a wild-type SaCas9 protein, wherein the modification comprises a mutation of at least one PAM-interacting residue, wherein the PAM-interacting residue is Y789, Y882, K886, N888, A889, L909, N985, N986, R991, or E993, and wherein the engineered SaCas9 protein is non-naturally occurring.
Dependencies are inferred from the claim text.