Method of preparing an artificial tooth primordium in vitro and artificial tooth primordium derived therefrom

A method of preparing an artificial tooth primordium in vitro, comprising the steps:

• providing isolated mesenchymal dental pulp cells in the form of a single-cell suspension;
• subsequently culturing the single-cell suspension of mesenchymal dental pulp cells under non-adherent conditions in ultra low attachment plates with a neutral hydrophilic hydrogel coated surface for at least two days to form a cell aggregate having a substantially spherical shape and a diameter of 0.3 mm to 2 mm,
• wherein no artificial biological or non-biological scaffold is used in the formation of the cell aggregate;
• transferring the mesenchymal dental pulp cell aggregates to a composite culture medium appropriate for both mesenchymal dental pulp cells and epithelial cells;
• adding a single cell suspension of epithelial cells in a ratio of 1:4 related to the initial cell number used for mesenchymal aggregate formation;
• culturing the cell mixture in ultra low attachment plates under non-adherent conditions;
• and changing the culture medium every 3 days.