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US 11,578,117 B2Granted
Antibodies and fragments thereof that bind hepatitis B virus protein X
AssigneeGilead Sciences, Inc.
InventorsSimon Paul Fletcher, Christian Alfons Voitenleitner
Life of this patent
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Abstract
Provided herein are, inter alia, antibodies, antigen-binding antibody fragments, cells, polynucleotides, compositions, kits, and methods relating to the detection of HBV protein X (HBx), e.g., in vitro and in vivo. Included are antibodies and fragments thereof that bind HBx, as well as kits, cells, and compositions comprising such antibodies and fragments.
Claims27
An isolated polynucleotide encoding an antibody, or an antigen-binding fragment thereof, comprising
- (i) a VH-complementarity determining region (CDR) 1 comprising
- the amino acid sequence of SEQ ID NO:1, or SEQ ID NO:1 having 1 conservative substitution,
- the amino acid sequence of SEQ ID NO:6, or SEQ ID NO:6 having 1 or 2 conservative substitutions,
- the amino acid sequence of SEQ ID NO:11, or SEQ ID NO:11 having 1 or 2 conservative substitutions, or
- the amino acid sequence of SEQ ID NO:15, or SEQ ID NO:15 having from 1 to 3 conservative substitutions;
- (ii) a VH-CDR2 comprising
- the amino acid sequence of SEQ ID NO:2, or SEQ ID NO:2 having from 1 to 4 conservative substitutions,
- the amino acid sequence of SEQ ID NO:7, or SEQ ID NO:7 having 1 or 2 conservative substitutions,
- the amino acid sequence of SEQ ID NO:12, or SEQ ID NO:12 having 1 conservative substitution, or
- the amino acid sequence of SEQ ID NO:16, or SEQ ID NO:16 having from 1 to 4 conservative substitutions;
- (iii) a VH-CDR3 comprising
- the amino acid sequence LAY,
- the amino acid sequence of SEQ ID NO:8, or SEQ ID NO:8 having 1 conservative substitution,
- an alanine residue, or
- the amino acid sequence LA;
- (iv) a VL-CDR1 comprising
- the amino acid sequence of SEQ ID NO:3, or SEQ ID NO:3 having from 1 to 4 conservative substitutions,
- the amino acid sequence of SEQ ID NO:9, or SEQ ID NO:9 having 1 or 2 conservative substitutions,
- the amino acid sequence of SEQ ID NO:13, or SEQ ID NO:13 having from 1 to 3 conservative substitutions, or
- the amino acid sequence of SEQ ID NO:17, or SEQ ID NO:17 having from 1 to 3 conservative substitutions;
- (v) a VL-CDR2 comprising
- the amino acid sequence of SEQ ID NO:4, or SEQ ID NO:4 having 1 or 2 conservative substitutions,
- the amino acid sequence KAS, or
- the amino acid sequence of SEQ ID NO:18, or SEQ ID NO:18 having from 1 to 3 conservative substitutions;
- and
- (vi) a VL-CDR3 comprising
- the amino acid sequence of SEQ ID NO:5, or SEQ ID NO:5 having 1 or 2 conservative substitutions,
- the amino acid sequence of SEQ ID NO:10, or SEQ ID NO:10 having 1 or 2 conservative substitutions,
- the amino acid sequence of SEQ ID NO:14, or SEQ ID NO:14 having 1 conservative substitution, or
- the amino acid sequence of SEQ ID NO:19, or SEQ ID NO:19 having 1 conservative substitution.
27 more elements
Dependencies are inferred from the claim text.