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US 11,576,394 B2Granted
Enzyme preparations yielding a clean taste
AssigneeDsm IP Assets B.V.
InventorsPetrus Jacobus Theodorus Dekker, Luppo Edens, Maximiliaan Peter Marie De Swaaf, Albertus Alard Van Dijk
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Abstract
The present invention describes a intracellular produced lactase, which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.
Claims7
A process for producing a lactase comprising
- (a) producing an arylsulfatase deficient yeast or fungal host cell using mutagenesis or recombinant genetic engineering to decrease the expression of a native arylsulfatase expression in the yeast or fungal host cell, wherein the arylsulfatase deficient host cell expresses less arylsulfatase than a parent yeast or fungal host cell not subjected to the mutagenesis or recombinant genetic engineering when cultured under the same conditions;
- (b) isolating the arylsulfatase deficient yeast or fungal host cell:
- (c) cultivating the arylsulfatase deficient yeast or fungal host cell in a nutrient medium under conditions resulting in expression of a lactase polypeptide:
- and
- (d) recovering the lactase polypeptide from the nutrient medium or from the yeast or fungal host cell.
2 more elements
Dependencies are inferred from the claim text.