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US 11,576,374 B2Granted
Capillary assisted vitrification processes and devices
AssigneeUpkara, Inc.
InventorsPravansu S. Mohanty, Nilay Chakraborty
Life of this patent
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Abstract
Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.
Claims11
A process for vitrification of one or more biological materials above cryogenic temperature of said biological material, the method comprising:
- a) providing a membrane comprising a plurality of contiguous capillary channels, each of said capillary channels having a first opening and a second opening;
- b) providing a vitrification mixture, said vitrification mixture comprising one or more biological materials and a vitrification medium;
- c) contacting said membrane with said vitrification mixture such that the membrane contacts a surface of the vitrification mixture, the membrane contacting the vitrification mixture such that the first opening of said capillary channels is operably in contact with said vitrification mixture;
- d) said second openings of said capillary channels directly operably in communication with a surrounding atmosphere having humidity below that of said vitrification mixture;
- and
- e) desiccating away the said vitrification mixture by capillary action above cryogenic temperature until the said vitrification mixture enters into a glassy state.
3 more elements
Dependencies are inferred from the claim text.