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US 11,573,200 B2Granted
Devices and methods for sample characterization
AssigneeIntabio, Inc.
InventorErik Gentalen
Life of this patent
Estimating the term…
Abstract
Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps.
Claims22
A method, comprising:
- (a) applying a first electric field across a first fluid channel in a microfluidic device to separate a mixture of charge variants of one or more biologics and ampholytes via isoelectric focusing while imaging the first fluid channel or a portion thereof;
- (b) applying a second electric field across the first fluid channel to mobilize the separated charge variants of one or more biologics while imaging the first fluid channel or a portion thereof;
- (c) expelling the mobilized charge variants of one or more biologics via electrospray ionization from an orifice in the microfluidic device into a mass spectrometer;
- and (d) correlating separated charge variants of one or more biologics peaks detected in (a) by the imaging of the first fluid channel or a portion thereof with mass spectrometer data for the separated charge variants of one or more biologics.
1 more element
A method, comprising:
- (a) separating a mixture of charge variants of one or more biologics in a first fluid channel in a microfluidic device while imaging the first fluid channel or a portion thereof;
- (b) mobilizing the separated charge variants of one or more biologics in the first fluid channel while imaging the first fluid channel or a portion thereof;
- (c) expelling the mobilized charge variants of one or more biologics via electrospray ionization from an orifice in the microfluidic device into a mass spectrometer;
- and (d) correlating separated charge variants of one or more biologics peaks detected in (a) by the imaging of the first fluid channel or a portion thereof with mass spectrometer data for the separated charge variants of one or more biologics.
1 more element
Dependencies are inferred from the claim text.