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US 11,573,182 B2Granted
Visualization, comparative analysis, and automated difference detection for large multi-parameter data sets
AssigneeFlowJo, LLC
InventorsMario Roederer, Michael D. Stadnisky
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Abstract
Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods for large, multi-parameter data sets. Frequency difference gating compares at least two different data sets to identify regions in a multivariate space where a frequency of events from a first data set is different than a frequency of events from the second data set according to a defined threshold.
Claims20
A computer-implemented method of visualizing differences between n-dimensional data sets, the computer-implemented method comprising:
- under control of one or more processing devices,
- receiving at least one threshold for frequency difference gating received data sets;
- receiving a first data set of n-dimensional data including a first plurality of flow cytometry events, corresponding to a first sample, in a plurality of first dimensions;
- receiving a second data set of n-dimensional data including a second plurality of flow cytometry events, corresponding to a second sample for cross-sample comparison with the first sample, in at least the plurality of first dimensions;
- concatenating the first data set and the second data set to obtain a concatenated data set;
- performing dimensionality reduction on the concatenated data set to obtain a mapping of events of the concatenated data set in a multivariate space defined by second dimensions different from and fewer than the plurality of first dimensions;
- identifying a frequency difference gate defining a population of events based at least in part on frequency difference gating performed on the mapping of events, the gate identifying a region in the multivariate space where a frequency of events from the first data set is different than a frequency of events from the second data set according to the at least one threshold;
- and
- generating a visualization from the frequency difference gated data for display via a display device, the visualization including a representation of events from the first data set and the second data set included in the population defined by the frequency difference gate, the visualization showing regions in the multivariate space where the frequency of events from the first data set is different than the frequency of events from the second data set according to the at least one threshold.
6 more elements
A system comprising:
- one or more processing devices;
- and
- a computer-readable storage medium comprising instructions that are configured to, when executed by the one or more processing devices, cause the system to:
- receive at least one threshold for frequency difference gating received data sets;
- receive a first data set of n-dimensional data including a first plurality of flow cytometry events, corresponding to a first sample, in a plurality of first dimensions;
- receive a second data set of n-dimensional data including a second plurality of flow cytometry events, corresponding to a second sample for cross-sample comparison with the first sample, in at least the plurality of first dimensions;
- concatenate the first data set and the second data set to obtain a concatenated data set;
- perform dimensionality reduction on the concatenated data set to obtain a mapping of events of the concatenated data set in a multivariate space defined by second dimensions different from and fewer than the plurality of first dimensions;
- identify a frequency difference gate defining a population of events based at least in part on frequency difference gating performed on the mapping of events, the gate identifying a region in the multivariate space where a frequency of events from the first data set is different than a frequency of events from the second data set according to the at least one threshold;
- and
- display a visualization including a representation of events from the first data set and the second data set included in the population defined by the frequency difference gate, the visualization showing regions in the multivariate space where the frequency of events from the first data set is different than the frequency of events from the second data set according to the at least one threshold.
8 more elements
Dependencies are inferred from the claim text.