Plants having increased tolerance to herbicides
AssigneeBASF Agro B.V.
InventorsRaphael Aponte, Stefan Tresch, Matthias Witschel, Jens Lerchl, Jill Marie Paulik, Chad Brommer
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Abstract
The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.
Claims16
An isolated nucleic acid comprising a nucleotide sequence encoding a mutated protoporphyrinogen oxidase (mut-PPO), wherein the encoded mut-PPO is a variant of SEQ ID NO:
- 46 having at least 75% identity to SEQ ID NO:
- 46 and wherein the encoded mut-PPO comprises an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine.
A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
- a) providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a mut-PPO, wherein the encoded mut-PPO is a variant of SEQ ID NO:
- 46 having at least 75% identity to SEQ ID NO:
- 46 and wherein the encoded mut-PPO comprises an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine;
- and
- b) applying to said site an effective amount of a benzoxazinone-derivative herbicide.
Dependencies are inferred from the claim text.