Regulating alkaloids
Assignee22nd Century Limited, LLC
InventorsTakashi Hashimoto, Akira Kato
Life of this patent
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Abstract
MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.
Claims8
A tobacco plant produced by a method comprising:
- (a) mutagenizing a plurality of tobacco plant cells to comprise one or more mutations in a nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 5, thereby producing a plurality of mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 5;
- (b) screening plants generated from the mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 5 for reduced expression of MPO1;
- and
- (c) selecting one or more of the progeny comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 5, wherein the progeny has reduced MPO1 expression and reduced nicotine content as compared to a control tobacco plant,
- thereby resulting in a tobacco plant having reduced MPO1 expression and reduced nicotine content as compared to a control non-mutagenized tobacco plant.
A tobacco plant comprising plant cells into which one or more mutations are introduced into a region of SEQ ID NO:
- 1 or SEQ ID NO:
- 5 that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, wherein the plant exhibits reduced expression of a gene product encoded by SEQ ID NO:
- 1 or SEQ ID NO:
- 5 as compared to a control non-mutagenized tobacco plant.
Dependencies are inferred from the claim text.