Methods and compositions involving promoters derived from Yarrowia lipolytica
AssigneeGinkgo Bioworks, Inc.
InventorsVasiliki Tsakraklides, Annapurna Kamineni
Life of this patent
Estimating the term…
Abstract
The current methods and compositions provide for nucleotide sequences of promoters from Yarrowia lipolytica which may be used to drive gene expression in a cell. In some aspects, the promoters are useful for modulating lipid production in oleaginous organisms such as yeast.
Claims22
A nucleic acid molecule comprising:
- a first sequence having at least 80% sequence identity to SEQ ID NO:1, 2, 5, 6 or 7, wherein the first sequence has promoter activity in a yeast cell;
- and
- a second sequence operably linked to the first sequence, wherein the second sequence is heterologous to the first sequence, and wherein the second sequence comprises a coding sequence.
A method of optimizing lipid production of a cell during a fermentation process, wherein the fermentation process comprises a growth phase and a lipid accumulation phase, the method comprising:
- (a) introducing into a cell a nucleic acid molecule comprising a promoter sequence having at least 80% sequence identity to SEQ IQ NO:
- 1, 2, 5, 6, or 7 to obtain a recombinant cell comprising the promoter sequence operably linked to a coding sequence, wherein the coding sequence is heterologous to the promoter sequence;
- and
- (b) culturing the recombinant cell.
A method of regulating the expression or activity of a coding sequence during a fermentation process wherein the fermentation process comprises a growth phase and a lipid accumulation phase, the method comprising:
- (a) introducing a nucleic acid molecule into a yeast cell, wherein the nucleic acid molecule comprises the coding sequence operably linked to a promoter sequence, wherein the coding sequence is heterologous to the promoter sequence, and wherein the promoter sequence has at least 80% sequence identity to SEQ ID NO:
- 1, 2, 5, 6, or 7;
- and
- (b) culturing the cell, thereby regulating expression or activity of the coding sequence.
Dependencies are inferred from the claim text.