Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
AssigneeChildren's Medical Center Corporation
InventorGeorge Q. Daley
Life of this patent
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Abstract
Embodiments herein relate to in vitro production methods of hematopoietic stem cell (HSC) and hematopoietic stem and progenitor cell (HSPC) that have long-term multilineage hematopoiesis potentials upon in vivo engraftment. The HSC and HSPCs are derived from pluripotent stem cells-derived hemogenic endothelia cells (HE) by non-integrative episomal vectors-based gene transfer.
Claims17
A method for making human hematopoietic stem cells (HSCs) and hematopoietic stem and progenitor cells (HSPCs) comprising in vitro transfecting human hemogenic endothelia cells (HE) with an exogenous gene coding copy of transcription factors ERG, HOXA9, HOXA5, LCOR and RUNX1 comprised in a non-integrative vector, wherein the transcription factors are expressed in the transfected cells to produce a population of multilineage HSCs and HSPCs that are capable of engrafting in a recipient host after implantation.
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