Well inserts with brittle membranes
AssigneeSimplinext SA
InventorsSilvia Angeloni Suter, Kaspar Suter, Eric Marguet, Sylvain Bergerat, Charlotte Voutat
Life of this patent
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Abstract
Disclosed is a well insert for cell culture, including: a membrane support having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein; and a permeable membrane for supporting a tissue culture, the permeable membrane being attached at the lower end of the membrane support and sealed thereto, the permeable membrane being of brittle material. The membrane support is overmolded or fastened on to the permeable membrane so as be sealed thereto.
Claims17
A well insert (10) for cell culture, comprising:
- a membrane support (10a) having an upper end and a lower end, said upper end being adapted to engage a well of a microplate so as to suspend the well insert (10) therein;
- and
- a permeable membrane (1), transparent in both air and water, for supporting a tissue culture, the permeable membrane (1) being attached at said lower end of the membrane support (10a) and sealed thereto, the permeable membrane (1) being ceramic and having a thickness less than 10 μm,
- wherein the membrane support (10a) is overmoulded on to the permeable membrane (1) such that the permeable membrane (1) is integrally moulded into a structure of the membrane support (10a),
- wherein the membrane support (10a) also has a first flange (16) in contact with a first side of the permeable membrane (1), and an opposing second flange (17) in contact with a second side of the permeable membrane (1), said second side being opposite said first side,
- wherein the membrane support (10a) is made of a polyolefin material.
Dependencies are inferred from the claim text.