Method for obtaining sterile human amniotic fluid and uses thereof
AssigneeMAM Holdings of West Florida, L.L.C.
InventorCarl Randall Harrell
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Abstract
Provided herein is a pre-Caesarean method for collecting amniotic fluid from a patient. A needle is inserted into the incision site for the future C-section, which may be under ultrasound guidance, through which the amniotic fluid is drawn under a low level suction and, optionally, gravity to a sterile collection container. The method encompasses filtering and/or irradiating the amniotic fluid to collect biomolecules of interest such as growth factors and/or stem cells. Also provided is the sterile amniotic fluid or filtrates thereof collected by the method described herein.
Claims10
A method for enhancing wound healing, cosmetic, orthopedic, or ophthalmic applications, comprising:
- administering an effective amount of a sterile filtered, cell-free, non-irradiated human amniotic fluid to a site in need thereof;
- wherein the sterile filtered, cell-free, non-irradiated human amniotic fluid comprises serially filtered human amniotic fluid prepared by a process comprising:
- collecting amniotic fluid under sterile conditions from a woman;
- removing cells, large particles, and other undissolvables from the human amniotic fluid by centrifuging the human amniotic fluid to obtain clarified amniotic fluid;
- and
- serially filtering the clarified amniotic fluid through multiple filters selected from the group consisting of depth filters and membrane filters with a pore size of 0.45 μm and 0.2 μm to obtain a sterile filtrate that is not irradiated.
Dependencies are inferred from the claim text.