Expression of PEDV sequences in plants and plant produced vaccine for same
AssigneeMazen Animal Health Inc.
InventorsJohn Howard, Erin Egelkrout, Celine Hayden
Life of this patent
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Abstract
A plant produced vaccine for Porcine Epidemic Diarrhea Virus (PEDV) is provided where the Spike protein of the virus is expressed in a plant by introducing into a plant a construct comprising a promoter preferentially directing expression to seed of said plant, a nucleoic acid encoding the Spike protein and a nucleic acid targeting expression to the endoplasmic reticulum of the plant. The plant expresses the S1 polypeptide at levels of at least 10 mg/kg of seed of said plant. When orally administered to an animal, a protective response is observed including a serum antibody response.
Claims18
A method of producing a protective response to Porcine Epidemic Diarrhea Virus (PEDV) in an animal, the method comprising,
- a) orally administering to said animal a composition comprising a plant or a plant product comprising the Spike (S1) protein of PEDV, said protein comprising SEQ ID NO:
- 3, 4, 9, 21 or 22 or a sequence having at least 90% identity to SEQ ID NO:
- 3, 4, 9, 21 or 22 or a functional fragment of said S1 protein expressed at levels of at least 10 mg/kg in seed of said plant, wherein said S1 protein is encoded by a nucleotide sequence comprising SEQ ID NO:
- 1, 2, or 25 or a sequence having at least 90% identity to SEQ ID NO:
- 1, 2, or 25;
- and
- b) producing a protective response to said PEDV in said animal.
A vaccine comprising a plant-produced polypeptide of the Spike (S1) protein, the vaccine comprising a plant or plant product comprising a construct comprising,
- (a) a promoter preferentially directing expression to seed tissue of a plant;
- (b) a nucleic acid molecule encoding a S1 polypeptide of said PEDV comprising SEQ ID NO:
- 3, 4 or 9 or a sequence having at least 90% identity to SEQ ID NO:
- 3, 4, 9, 21 or 22 or a functional fragment operably linked to said promoter, wherein said S1 protein is encoded by a nucleotide sequence comprising SEQ ID NO:
- 1, 2, or 25 or a sequence having at least 90% identity to SEQ ID NO:
- 1, 2, or 25;
- and
- (c) a nucleic acid molecule targeting expression of said polypeptide in the endoplasmic reticulum of said plant;
- and expressing said S1 polypeptide in said plant at levels of at least 1 mg/kg of seed of said plant.
A method of expressing a polypeptide of Spike (S1) protein or functional fragment thereof of Porcine Epidemic Diarrhea Virus (PEDV) the method comprising, introducing into a plant a construct comprising,
- (a) a promoter preferentially directing expression to seed tissue of a plant;
- (b) a nucleic acid molecule encoding a S1 polypeptide of said PEDV comprising SEQ ID NO:
- 3, 4 or 9 or a sequence having at least 90% identity to SEQ ID NO:
- 3, 4, 9, 21 or 22 or a functional fragment operably linked to said promoter, wherein S1 protein is encoded by a nucleotide sequence comprising SEQ ID NO:
- 1, 2, or 25 or a sequence having at least 90% identity to SEQ ID NO:
- 1, 2, or 25;
- and
- (c) a nucleic acid molecule targeting expression of said polypeptide in the endoplasmic reticulum of said plant;
- and expressing said S1 polypeptide in said plant at levels of at least 1 mg/kg of seed of said plant.
Dependencies are inferred from the claim text.