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US 11,566,240 B2Granted
Desorption of nucleic acids
AssigneeRobert Bosch GmbH
InventorsJochen Rupp, Christian Dorrer, Juergen Steigert, Bernd Faltin, Karsten Seidl
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Abstract
In a method for the desorption of nucleic acids from a sample, in order to simplify the desorption of nucleic acids from the sample, a solid phase is repeatedly rinsed with an elution buffer in a microfluidic system, in order to elute nucleic acids bonded to the solid phase from the solid phase in the microfluidic system.
Claims11
A method for the desorption of nucleic acids from a sample, comprising:
- rinsing a solid phase with a first fraction volume of elution buffer in a microfluidic system;
- moving the first fraction volume to a collection chamber after rinsing the solid phase the first time;
- rinsing the solid phase multiple times with a second fraction volume of elution buffer the microfluidic system, after rinsing the solid phase with the first fraction volume, such that nucleic acids bound to the solid phase are eluted from the solid phase in the microfluidic system, wherein the elution is effected by a micropump integrated into the microfluidic system and having a defined displacement volume that corresponds to an elution volume.
Dependencies are inferred from the claim text.