Kit containing medium for culturing natural killer cells and method of effectively culturing natural killer cells using the same

A method of culturing natural killer cells using a kit containing a medium for culturing cells comprising:

• a basic solution containing L-glutamine, IL-2 and a medium for cell culture;
• a C-1 solution containing IL-12 in the basic solution;
• a C-2 solution containing IL-15 in the basic solution;
• a C-3 solution containing IL-17 and IL-21 in the basic solution;
• a C-4 solution containing IL-18 in the basic solution;
• an A-1 solution containing an anti-CD16 antibody and an anti-CD-56 antibody in the basic solution;
• and an A-2 solution containing an anti-CD3 antibody in the basic solution,
• the method comprising:
• a first step comprising adding said basic solution and said A-1 solution to separated lymphocytes and further adding autologous plasma thereto to stimulate NK cells;
• a second step of adding said A-2 solution and autologous plasma thereto to accelerate initial proliferation of the NK cells;
• a third step of adding said C-1, C-2, C-3 and C-4 solutions and autologous plasma thereto to amplify culture of the NK cells;
• and a fourth step of amplifying and culturing the NK cells in the basic solution and the autologous plasma.