Dendritic cell generator
AssigneeNortheastern University
InventorsShashi K. Murthy, Bradley B. Collier
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Abstract
Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.
Claims20
A method for dendritic cell generation, the method comprising:
- providing a cell culture chamber comprising a fluidic channel and an inlet;
- introducing a liquid sample containing monocytes into the fluidic channel via the inlet;
- and
- flowing a fluid through the fluidic channel such that a flow rate of the fluid through the fluidic channel is less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel, thereby maintaining the monocytes and dendritic cells within the fluidic channel without use of filters.
A method for dendritic cell generation, the method comprising:
- introducing a liquid sample containing monocytes into a fluidic channel of a cell culture chamber via an inlet of the cell culture chamber;
- and
- flowing a fluid through the fluidic channel such that a flow rate of the fluid through the fluidic channel is less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel.
A method for dendritic cell generation, the method comprising:
- causing a fluid to flow through a fluidic channel of a cell culture chamber comprising monocytes introduced into the fluidic channel via an inlet of the cell culture chamber, the fluid being caused to flow at a flow rate of less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel.
Dependencies are inferred from the claim text.