Photo-chemical synthesis and characterization of silver nanoparticles self-arranged on ct-DNA and BSA
AssigneeYildiz Teknik Universitesi
InventorsNergis Arsu, Eyup Metin, Gonul Saadet Batibay, Nur Sena Okten
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Abstract
The present invention relates to examination of characteristics of silver nano-clusters self-arranged by means of reduction of silver salt to silver nanoparticles by means of 2-mercapto-thioxanthone on DNA and BSA in short photo-illumination duration of 1 second.
Claims5
A method for obtaining self-arranged silver nanoparticles in which color change is observed after a photo-illumination duration of 1 second on ct-DNA and which is compliant for usage in development of imaging methods for diagnosis and treatment purposes, the method comprising:
- a) Adding a ct-DNA solution having a concentration of ct-DNA in a range from 1×10−5 M and 1×10−4 M, a 2-mercapto thioxanthone solution having a concentration in a range from 0.5×10−3 M and 3×10−3 M, and a Tris-HCl solution to an AgNO3 solution having a concentration in a range from 0.5×10−3 M and 3×10−3;
- b) mixing the mixture formed in step a) until it becomes homogeneous;
- c) subjecting the mixture to an UV light source at a distance between 1-10 cm;
- d) taking UV-Vis absorption spectrum and fluorescence emission spectrum simultaneously in the duration of subjecting to light source;
- e) concluding that the silver nanoparticles and/or silver nano-clusters are formed when peak is observed between 400 and 450 nm in UV-Vis spectrum.
A method for obtaining self-arranged silver nanoparticles on BSA and which is compliant for usage in development of imaging methods for diagnosis and treatment purposes, characterized by comprising the process steps of:
- a) Adding a BSA solution having a concentration of BSA in a range from 0.5×10−3 M and 3×10−3 M, a 2-mercapto thioxanthone solution having a concentration of 2-mercapto thioxanthone in a range from 0.5×10−3 M and 3×10−3 M, and a Tris-HCl solution to an AgNO3 solution having a concentration of AgNO3 in a range from 0.5×10−3M and 3×10−3 M;
- b) mixing the mixture formed in step a) until it becomes homogeneous;
- c) subjecting the mixture to an UV light source at a distance between 1-10 cm;
- d) taking UV-Vis absorption spectrum and fluorescence emission spectrum simultaneously in the duration of subjecting to light source,
- e) monitoring formation of Ag nanoparticles (AgNP) and afterwards Ag nano-clusters (AgNC) from the absorption graphic.
Dependencies are inferred from the claim text.