Multi-chambered assay devices and associated methods, systems and apparatuses thereof for detection of analytes

An assay device configured for arrangement on a disc, and configured to process an individual sample, the assay device positioned along a periphery of the disc at a predetermined radius in a spaced apart arrangement, the assay device comprising:

• a plurality of chambers each configured to receive one or more fluids via a respective inlet area;
• a resuspension chamber including a scaffold for drying and retaining at least one reagent;
• a main chamber comprising a mixing area;
• at least one bead;
• and
• a bead stabilization area, separate from the mixing area and main chamber, wherein the bead stabilization area:
• is arranged between an end of the mixing area and an outer edge of the disc,
• is in fluid communication with the mixing area and includes an opening for receiving the at least one bead from the mixing area, and
• is sized and shaped so as to contain the at least one bead when at rest so as to remove it from the mixing area.