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US 11,565,240 B2Granted
Mixed mode affinity chromatography carrier
AssigneeFUJIFILM Corporation
InventorShiori Kihara
Life of this patent
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Abstract
A mixed mode affinity chromatography carrier includes a substrate, a hydrophilic polymer, an antibody-binding cyclic peptide, and a cation exchange group.
Claims18
A mixed mode affinity chromatography carrier, comprising:
- a substrate that is at least one selected from the group consisting of a polysaccharide, an acrylate-based polymer, a methacrylate-based polymer, and a styrene-based polymer;
- a hydrophilic polymer coated on the substrate, the hydrophilic polymer being a hydrophilic polysaccharide;
- an antibody-binding cyclic peptide that includes a cyclic portion cyclized by intramolecular crosslinking between side chains;
- and
- a cation exchange group that is a carboxy group or a sulfoxy group.
2 more elements
A mixed mode affinity chromatography carrier, comprising:
- a substrate that is at least one selected from the group consisting of a polysaccharide, an acrylate-based polymer, a methacrylate-based polymer, and a styrene-based polymer;
- a hydrophilic polymer that is a hydrophilic polysaccharide;
- an antibody-binding cyclic peptide that includes a cyclic portion cyclized by intramolecular crosslinking between side chains;
- and
- a cation exchange group that is a carboxy group or a sulfoxy group,
- wherein the intramolecular crosslinking includes at least one selected from the group consisting of a disulfide bond, a thioether bond, and a triazole bond,
- the disulfide bond is a disulfide bond formed between a side chain thiol group of a first amino acid residue derived from an amino acid having a thiol group in a side chain other than L-cysteine and D-cysteine and a side chain thiol group of a second amino acid residue derived from an amino acid having a thiol group in a side chain other than L-cysteine and D-cysteine, and
- wherein the thioether bond is a thioether bond formed between a side chain thiol group of a first amino acid residue derived from an amino acid having a thiol group in a side chain other than L-cysteine and D-cysteine and a side chain haloacetyl group of a second amino acid residue derived from an amino acid having a haloacetyl group in a side chain.
5 more elements
A mixed mode affinity chromatography carrier, comprising:
- a substrate that is at least one selected from the group consisting of a polysaccharide, an acrylate-based polymer, a methacrylate-based polymer, and a styrene-based polymer;
- a hydrophilic polymer that is a hydrophilic polysaccharide;
- an antibody-binding cyclic peptide that includes a cyclic portion cyclized by intramolecular crosslinking between side chains;
- and
- a cation exchange group that is a carboxy group or a sulfoxy group,
- wherein the intramolecular crosslinking includes at least one selected from the group consisting of a disulfide bond, a thioether bond, and a triazole bond,
- wherein the disulfide bond is a disulfide bond formed between a side chain thiol group of a first amino acid residue derived from an amino acid having a thiol group in a side chain selected from the group consisting of cysteine, homocysteine, and penicillamine and a side chain thiol group of a second amino acid residue derived from an amino acid having a thiol group in a side chain selected from the group consisting of cysteine, homocysteine, and penicillamine, and
- wherein the thioether bond is a thioether bond formed between a side chain thiol group of a first amino acid residue derived from an amino acid having a thiol group in a side chain selected from the group consisting of cysteine, homocysteine, and penicillamine and a side chain haloacetyl group of a second amino acid residue derived from an amino acid having a haloacetyl group in a side chain.
5 more elements
Dependencies are inferred from the claim text.