Methods for generating universal and custom MHC/HLA-compatible hematopoietic progenitor cells

A method of treating a pathogen infection in a subject in need thereof, said method comprising:

• a) generating custom MHC/HLA-compatible hematopoietic progenitor cells for the subject, said generating comprising the steps of:
• i) a contacting step, wherein the contacting step consists of contacting isolated MHC/HLA-compatible progenitor cells with a fusion protein consisting of a N-terminal cell-penetration peptide and a C-terminal a mixed-lineage leukemia (MLL) oncoprotein, the fusion protein having the sequence of SEQ ID NO:
• 1;
• wherein said isolated progenitor cells are:
• progenitor cells that give rise to subsets of mature blood cells;
• and isolated from bone marrow, peripheral blood, placenta, or umbilical cord of a donor subject;
• and
• ii) a culturing step, wherein the culturing step comprises the progenitor cells of step i) with a combination of multilineage cytokines comprising stem-cell factor (SCF), Flt3 ligand, IL-3, TPO and IL-6, whereupon culturing, the progenitor cells become immortalized and exhibit commitment to neutrophil, macrophage, and/or dendritic lineage or exhibit multi-lineage blood cell differentiation potential;
• b) administering the cells resulting from step a) to the subject.