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US 11,561,219 B2Granted
Methods of enumerating particles present in a cell composition
AssigneeJuno Therapeutics, Inc.
InventorsKien Khuu-Duong, Calvin Chan, Rachel K. Yost, Brian Christin, Ruth Berry, Janelle Stoops
Life of this patent
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Abstract
Provided herein are methods of assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.
Claims26
A method for enumerating or detecting the presence or absence of residual bead particles in a cell composition, the method comprising the steps of:
- a) incubating a sample comprising at least a portion of the cell composition that is suspected of comprising residual bead particles with a hypotonic solution under a condition sufficient to induce osmotic lysis of one or more cells in the sample to produce a lysed cell composition, and
- incubating at least a portion of the lysed cell composition with a hypertonic solution;
- and
- b) determining the presence, absence, number and/or concentration of residual bead particles in the output composition, thereby enumerating or detecting the presence or absence of residual bead particles in the cell composition.
1 more element
A method of enumerating or detecting the presence or absence of residual bead particles in a cell composition, the method comprising the steps of:
- a) incubating a sample comprising at least a portion of the cell composition that is suspected of comprising residual bead particles with a hypotonic solution under a condition sufficient to induce lysis of one or more cells in the sample to produce a lysed cell composition, and
- incubating at least a portion of the lysed cell composition with a hypertonic solution;
- and
- b) determining the presence, absence, number and/or concentration of residual bead particles in the output composition using a binding agent that specifically binds to a material or a biomolecule present on, associated with or attached to the residual bead particle, thereby enumerating or detecting the presence or absence of residual bead particles in the cell composition.
1 more element
Dependencies are inferred from the claim text.