Methods of producing lipid-derived compounds and host cells thereof
AssigneeNational Technology & Engineering Solutions of Sandia, LLC
InventorsSamuel Coradetti, John Michael Gladden, Di Liu, Gina Marie Geiselman
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Abstract
The present disclosure relates to genetically engineered host cells and methods of producing a lipid-derived compound by employing such host cells. In particular embodiments, the host cell includes a first mutant gene encoding a cytoplasmic tRNA thiolation protein. Optionally, the host cell can include other mutant genes for decreasing fatty alcohol catabolism, decreasing re-importation of secreted fatty alcohol, or displaying other useful characteristics, as described herein.
Claims15
An isolated, genetically engineered host cell comprising:
- a first mutant gene that is a deletion of a gene encoding a cytoplasmic tRNA thiolation protein;
- and
- one or more expressed nucleic acids encoding 1) a fatty acyl-CoA reductase, or 2) a thioesterase, a carboxylicacid reductase and an aldehyde reductase.
A method of producing a fatty alcohol, the method comprising:
- incubating an isolated, genetically engineered host cell in a culture;
- and
- isolating one or more fatty alcohols from the culture,
- wherein the genetically engineered host cell comprises a first mutant gene that is a deletion of a gene encoding a cytoplasmic tRNA thiolation protein, and further one or more expressed nucleic acids encoding 1) a fatty acyl-CoA reductase, or 2) a thioesterase, a carboxylic acid reductase and an aldehyde reductase.
Dependencies are inferred from the claim text.