Target binding moiety compositions and methods of use
AssigneeRootPath Genomics, Inc.
InventorsXi Chen, Ely Porter
Life of this patent
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Abstract
Provided herein are compositions and methods to identify a binding element (e.g., peptide, peptoid, or protein) that can be bound by an immunoreceptor (e.g., antibody). The binding element can be provided in a target binding unit comprising two binding elements separated by a spacer such that the two binding elements simultaneously bind to a single molecule comprising an antigen binding domain of an antibody. The present disclosure provides various methods to construct the spacer. The identified binding elements can be further used to manufacture an array which can be used to profile antibodies obtained from a blood sample.
Claims20
A composition comprising a polynucleotide-barcoded target binding moiety, wherein the polynucleotide-barcoded target binding moiety comprises
- (a) a nucleic acid sequence linked by a linker to
- (b) a target binding unit comprising
- (i) a first peptide sequence comprising a first binding region, and
- (ii) a second peptide sequence comprising a second binding region;
- wherein the first binding region and the second binding region are
- (i) separated by a spacer, and
- (ii) spaced at a distance such that the first peptide sequence and the second peptide sequence simultaneously bind to a single molecule comprising an antigen binding domain of an antibody;
- wherein the nucleic acid sequence encodes the first peptide sequence and/or the second peptide sequence;
- and
- wherein the composition is soluble.
A method comprising:
- contacting a mixture of antibodies with a population of polynucleotide-barcoded target binding moieties, wherein each target binding moiety of the population comprises a target binding unit having a first binding region and a second binding region, wherein the first binding region and the second binding region are separated by a spacer and spaced at a distance such that the first binding region and the second binding region simultaneously bind to a single antibody molecule of the mixture.
Dependencies are inferred from the claim text.