Broad-spectrum monoclonal antibodies against chikungunya virus E1 structural protein

An antibody against Chikungunya virus or an antigen-binding fragment of the antibody, comprising the following heavy chain variable region (1), (2), or (3) and the following light chain variable region (4):

• (1) a heavy chain variable region comprising a polypeptide consisting of the following amino acid sequence (H-A) that is selected from the group consisting of amino acid sequences (H-A1), (H-A2), and (H-A3):
• (H-A1) an amino acid sequence of SEQ ID NO:
• 13,
• (H-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 13, and
• (H-A3) an amino acid sequence in which one to twenty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 13,
• wherein each of (H-A1), (H-A2), and (H-A3) comprises sequences of heavy chain complementarity determining regions (CDRHA)-1 comprising an amino acid sequence of SEQ ID NO:
• 1, CDRHA-2 comprising an amino acid sequence of SEQ ID NO:
• 2, and CDRHA-3 comprising an amino acid sequence of SEQ ID NO:
• 3,
• wherein the CDRHA-1, the CDRHA-2, and the CDRHA-3 sequences are invariant, (2) a heavy chain variable region comprising a polypeptide consisting of the following amino acid sequence (H-B) that is selected from the group consisting of amino acid sequences (H-B1), (H-B2), and (H-B3):
• (H-B1) an amino acid sequence of SEQ ID NO:
• 14,
• (H-B2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 14, and
• (H-B3) an amino acid sequence in which one to twenty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 14,
• wherein each of (H-B1), (H-B2), and (H-B3) comprises sequences of CDRHB-1 comprising an amino acid sequence of SEQ ID NO:
• 4, CDRHB-2 comprising an amino acid sequence of SEQ ID NO:
• 5, and CDRHB-3 comprising an amino acid sequence of SEQ ID NO:
• 6,
• wherein the CDRHB-1, the CDRHB-2, and the CDRHB-3 sequences are invariant, (3) a heavy chain variable region comprising a polypeptide consisting of the following amino acid sequence (H-C) that is selected from the group consisting of
• amino acid sequences (H-C1), (H-C2), and (H-C3):
• (H-C1) an amino acid sequence of SEQ ID NO:
• 15,
• (H-C2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 15, and
• (H-C3) an amino acid sequence in which one to twenty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 15,
• wherein each of (H-C1), (H-C2), and (H-C3) comprises sequences of CDRHC-1 comprising an amino acid sequence of SEQ ID NO:
• 7, CDRHC-2 comprising an amino acid sequence of SEQ ID NO:
• 8, and CDRHC-3 comprising an amino acid sequence of SEQ ID NO:
• 9,
• wherein the CDRHC-1, the CDRHC-2, and the CDRHC-3 sequences are invariant, (4) a light chain variable region comprising a polypeptide consisting of the following amino acid sequence (L-A) that is selected from the group consisting of
• amino acid sequences (L-A1), (L-A2), and (L-A3):
• (L-A1) an amino acid sequence of SEQ ID NO:
• 16,
• (L-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 16, and
• (L-A3) an amino acid sequence in which one to twenty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 16,
• wherein each of (L-A1), (L-A2), and (L-A3) comprises sequences of light chain complementarity determining regions (CDRL)-1 comprising an amino acid sequence of SEQ ID NO:
• 10, CDRL-2 comprising an amino acid sequence of SEQ ID NO:
• 11, and CDRL-3 comprising an amino acid sequence of SEQ ID NO:
• 12,
• wherein the CDRL-1, the CDRL-2, and the CDRL-3 sequences are invariant.

An antibody against Chikungunya virus or an antigen-binding fragment of the antibody, comprising the following heavy chain (1), (2), or (3) and the following light chain (4):

• (1) a heavy chain comprising a polypeptide consisting of the following amino acid sequence (HA) that is selected from the group consisting of
• amino acid sequences (HA1), (HA2), and (HA3):
• (HA1) an amino acid sequence of SEQ ID NO:
• 17,
• (HA2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 17, and
• (HA3) an amino acid sequence in which one to eighty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 17,
• wherein the SEQ ID NO:
• 17 comprises sequences of heavy chain complementarity determining regions (CDRHA)-1 comprising an amino acid sequence of SEQ ID NO:
• 1, CDRHA-2 comprising an amino acid sequence of SEQ ID NO:
• 2, and CDRHA-3 comprising an amino acid sequence of SEQ ID NO:
• 3,
• wherein the CDRHA-1, the CDRHA-2, and the CDRHA-3 sequences are invariant, (2) a heavy chain comprising a polypeptide consisting of the following amino acid sequence (HB) that is selected from the group consisting of
• amino acid sequences (HB1), (HB2), and (HB3):
• (HB1) an amino acid sequence of SEQ ID NO:
• 18,
• (HB2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 18, and
• (HB3) an amino acid sequence in which one to eighty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 18,
• wherein the SEQ ID NO:
• 18 comprises sequences of CDRHB-1 comprising an amino acid sequence of SEQ ID NO:
• 4, CDRHB-2 comprising an amino acid sequence of SEQ ID NO:
• 5, and CDRHB-3 comprising an amino acid sequence of SEQ ID NO:
• 6,
• wherein the CDRHB-1, the CDRHB-2, and the CDRHB-3 sequences are invariant,
• (3) a heavy chain comprising a polypeptide consisting of the following amino acid sequence (HC) that is selected from the group consisting of
• amino acid sequences (HC1), (HC2), and (HC3):
• (HC1) an amino acid sequence of SEQ ID NO:
• 19,
• (HC2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 19, and
• (HC3) an amino acid sequence in which one to eighty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 19,
• wherein the SEQ ID NO:
• 19 comprises sequences of CDRHC-1 comprising an amino acid sequence of SEQ ID NO:
• 7, CDRHC-2 comprising an amino acid sequence of SEQ ID NO:
• 8, and CDRHC-3 comprising an amino acid sequence of SEQ ID NO:
• 9,
• wherein the CDRHC-1, the CDRHC-2, and the CDRHC-3 sequences are invariant, (4) a light chain comprising a polypeptide consisting of the following amino acid sequence (LA) that is selected from the group consisting of
• amino acid sequences (LA1), (LA2), and (LA3):
• (LA1) an amino acid sequence of SEQ ID NO:
• 20,
• (LA2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
• 20, and
• (LA3) an amino acid sequence in which one to forty amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
• 20,
• wherein SEQ ID NO:
• 20 comprises sequences of light chain complementarity determining regions (CDRL)-1 comprising an amino acid sequence of SEQ ID NO:
• 10, CDRL-2 comprising an amino acid sequence of SEQ ID NO:
• 11, and CDRL-3 comprising an amino acid sequence of SEQ ID NO:
• 12,
• wherein the CDRL-1, the CDRL-2, and the CDRL-3 sequences are invariant.