Process for the production of sulfated phenolic compounds using modified Escerichia coli
AssigneeCysBio ApS
InventorsChristian Bille Jendresen, Alex Toftgaard Nielsen
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Abstract
The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using recombinant host cells. More particularly, the present invention pertains to recombinant host cells comprising (e.g., expressing) a polypeptide having aryl sulfotransferase activity, wherein said recombinant host cells have been modified to have an increased uptake of sulfate compared to identical host cells that does not carry said modification. Further provided are processes for the production of aryl sulfates, such as zosteric acid, employing such recombinant host cells.
Claims22
A process for the production of a sulfated phenolic compound comprising:
- (i′) contacting a medium comprising a phenolic compound or a precursor of a phenolic compound with a first recombinant host cell;
- wherein the first recombinant host cell comprises a heterologous polypeptide having an aryl sulfotransferase activity, and wherein the first recombinant host cell has been modified to have an increased protein expression of a sulfate transporter compared to the identical host cell that does not carry said modification;
- or
- (i″) contacting a medium comprising a fermentable carbon substrate with a first recombinant host cell, a phenolic compound being produced from the fermentable carbon substrate by the first recombinant host cell;
- wherein the first recombinant host cell comprises a heterologous polypeptide having an aryl sulfotransferase activity, and wherein the first recombinant host cell has been modified to have an increased protein expression of a sulfate transporter compared to the identical host cell that does not carry said modification;
- wherein the polypeptide having an aryl sulfotransferase activity comprises an amino acid sequence which has at least 95% sequence identity with the amino acid sequence set forth in SEQ ID NO:
- 1, 6, 7, 8 or 13;
- and
- wherein the sulphate transporter is a CysP transporter belonging to the phosphate inorganic transporter (PiT) family.
Dependencies are inferred from the claim text.