Pipette tip and uses and methods thereof

A pipette tip comprising a constituent (A) and a constituent (B), the pipette tip having:

• an open upper end configured to fit to an end of a pipette;
• an open lower end configured to aspirate and release a liquid sample;
• and
• a longitudinal axis spanning a length from the open upper end to the open lower end;
• wherein a first region of the pipette tip along the longitudinal axis comprises constituent (A) and a second region of the pipette tip along the longitudinal axis comprises constituent (B),
• said first region being selected from the group consisting of a first circumferential reagent layer deposited on a first circumferential inner surface area of the pipette tip and a first porous insert contained by the first circumferential inner surface area of the pipette tip,
• said second region being selected from the group consisting of a second circumferential reagent layer deposited on a second circumferential inner surface area of the pipette tip and a second porous insert contained by the second circumferential inner surface area of the pipette tip,
• constituent (A) being physically confined and releasably coupled to one of the first region and the second region and being separate from constituent (B) along the longitudinal axis of the pipette tip, and
• constituent (B) being physically confined and releasably coupled to the other one of the first region and the second region and being separate from constituent (A) along the longitudinal axis of the pipette tip,
• wherein:
• (a) the first circumferential inner surface area and the second circumferential inner surface area are non-overlapping along the longitudinal axis of the pipette tip, and
• (b) constituent (A) is different than constituent (B);
• and
• (c) constituent (A) and constituent (B) are configured to form a diagnostic composition upon their release from the first region and the second region.