Genetic perturbation of the RNA degradosome protein complex
AssigneeZymergen Inc.
InventorShawn Manchester
Life of this patent
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Abstract
The present disclosure provides novel bacterial strains with altered expression or start codon modification of one or more RNA degradation/processing genes. The RNA degradation genes of the present disclosure are controlled by heterologous promoters. The present disclosure further describes methods for generating microbial strains comprising heterologous promoter sequences operably linked to RNA degradation/processing genes.
Claims40
A genetically engineered prokaryotic host cell with enhanced industrial performance, said host cell comprising:
- a. a heterologous promoter polynucleotide, and
- b. a polynucleotide encoding an RNA degradation gene;
- wherein the heterologous promoter polynucleotide is operably linked to the polynucleotide encoding the RNA degradation gene, wherein the heterologous promoter comprises a promoter from a promoter ladder;
- wherein the polynucleotide encoding the RNA degradation gene comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 20, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 17, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 9, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 21, and SEQ ID NO:
- 22.
A genetically engineered prokaryotic host cell with enhanced industrial performance, said host cell comprising:
- a. a heterologous promoter polynucleotide, and
- b. a polynucleotide encoding an RNA degradation gene;
- wherein the heterologous promoter polynucleotide is operably linked to the polynucleotide encoding the RNA degradation gene, wherein the heterologous promoter comprises a promoter from a promoter ladder;
- wherein the heterologous promoter is a promoter comprising a nucleotide sequence selected from the group consisting of SEQ ID Nos:
- 1-8.
A genetically engineered prokaryotic host cell with enhanced industrial performance, said host cell comprising:
- a. a heterologous promoter polynucleotide, and
- b. a polynucleotide encoding an RNA degradation gene;
- wherein the heterologous promoter is derived from Corynebacterium glutamicum, is less than 100 base pairs in length, is able to constitutively express genes across different growth conditions, and is able to co-operate to form a ladder of promoters comprising a plurality of promoters with incrementally increasing levels of promoter activity.
Dependencies are inferred from the claim text.