Intratumoral vaccination

A method for treating a tumor in a subject in need thereof, comprising intratumorally delivering an effective amount of a composition comprising an expression vector that comprises a first nucleotide sequence encoding a secretable gp96-Immunoglobulin fusion protein which lacks the gp96 KDEL (SEQ ID NO:3) sequence, and a second nucleotide sequence encoding a T cell costimulatory fusion protein, by electroporation wherein the T cell costimulatory fusion protein is OX40L-Ig, or a portion thereof that binds to OX40, wherein the intratumoral delivery is in vivo by injection, and further administering to the subject an effective amount of a biological cell comprising an expression vector that comprises a first nucleotide sequence encoding a secretable gp96-Ig fusion protein which lacks the gp96 KDEL (SEQ ID NO:3) sequence, and a second nucleotide sequence encoding a T cell costimulatory fusion protein, wherein the T cell costimulatory fusion protein is OX40L-Ig, or a portion thereof that binds to OX40, and the T cell costimulatory fusion protein enhances activation of antigen-specific T cells when administered to the subject.