Nanonets and spherical particles
AssigneeThe James Hutton Institute
InventorsAndrew John Love, Mikhail Emmanuilovich Talianski, Kara McGeachy
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Abstract
The present invention relates to macromolecular complexes comprising micron-scale networks which include binding motifs thereon which allow the covalent bonding of the micron-scale networks to particles which provide nanoscale display surfaces. In particular the present invention relates to micron-scale networks of TMV coat proteins comprising a peptide tag (e.g. SpyTag) and particles providing a nanoscale display surface comprising GFP and a corresponding binding protein (e.g. SpyCatcher) wherein the peptide tag and binding protein pair are capable of spontaneously forming a covalent bond.
Claims17
A macromolecular complex comprising a first and second complex
- wherein
- a) the first complex comprises a virus derived self-assembled micron scale macromolecular network structure comprising a tobacco mosaic virus (TMV) coat protein which surface displays a motif for one of a peptide tag and binding protein pair that can spontaneously form covalent linkages with the second complex wherein the peptide tag and binding protein pair are SpyTag and SpyCatcher, and
- b) the second complex comprises a particle which surface displays a motif interacting partner encoding one of the corresponding peptide tag and binding protein pair, wherein the first complex is covalently linked to the second complex.
A nanoscale surface display system which binds to a first complex comprising a virus derived self-assembled micron scale macromolecular network structure comprising a tobacco mosaic virus (TMV) coat protein, wherein the virus derived self-assembled micron scale structure comprises a first member of a covalently reactive peptide tag and binding protein pair, wherein the peptide tag and binding protein pair are SpyTag and SpyCatcher, wherein the peptide tag and binding protein pair are capable of spontaneously forming a covalent bond, the nanoscale display surface comprising a second member of a covalently reactive peptide tag and binding protein pair that spontaneously forms a covalent bond with the first member.
A virus derived self-assembled micron scale macromolecular network structure comprising a tobacco mosaic virus (TMV) coat protein, wherein the micron scale macromolecular network structure is capable of binding to a second complex comprising a nanoscale display surface, the nanoscale display surface comprising a second member of a covalently reactive peptide tag and binding protein pair, wherein the peptide tag and binding protein pair are SpyTag and SpyCatcher, wherein the micron scale structure comprises a first member of a covalently reactive peptide tag and binding protein pair wherein the peptide tag and binding protein pair are capable of spontaneously forming a covalent bond.
Dependencies are inferred from the claim text.