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US 11,547,099 B2Granted
Transgenic mice
AssigneeCrescendo Biologies Limited
InventorsYumin Teng, Joyce Young, Brian McGuinness, Mike Romanos, Marianne Brueggemann
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Abstract
The invention relates to nucleic acid constructs for expression in mice for the production of heavy chain only antibodies and VH domains, transgenic mice, related methods and uses.
Claims11
A transgenic mouse comprising in its genome a vector comprising:
- a) at least 10 functional human heavy chain V genes wherein at least 10 functional human heavy chain V genes are in their natural configuration;
- b) at least one human heavy chain D gene and at least one human heavy chain J gene;
- c) a murine μ enhancer;
- d) a murine switch μ element;
- e) a murine C gene which lacks the CH1 exon, wherein the C gene consists of a C gene selected from Cγ1, Cγ2b and/or Cγ2a;
- and
- f) a murine 3′ enhancer region,
- wherein Cμ is absent in the mouse, or Cμ is partially absent to the extent that it is non-functional, and
- wherein the transgenic mouse is capable of expressing a chimeric immunoglobulin heavy chain encoded by the vector in response to antigen challenge.
6 more elements
Dependencies are inferred from the claim text.