Kit and method for quantitative detection of HBsAg
AssigneesXiamen Innodx Biotech Co., Ltd, Xiamen University
InventorsShunhua Wen, Liuwei Song, Zuxing Weng, Haijun Lin, Feihai Xu, Xudong Sun, Shengxiang Ge
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Abstract
A kit for quantitatively detecting HBsAg and a method for quantitatively detecting an HBsAg content in a sample containing HBsAg. The kit comprises a first antibody specifically binding to HBsAg and a reagent composition. The reagent composition comprises tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and urea.
Claims18
A kit, comprising a first antibody capable of specifically binding to HBsAg, and a reagent composition, the reagent composition comprising tris(2-carboxyethyl)phosphine hydrochloride (TCEP), urea, a non-ionic surfactant, an inorganic salt and a buffer, wherein:
- the TCEP is present in an amount of 1 to 100 mM;
- the urea is present in an amount of 0.5 to 8 M;
- the non-ionic surfactant is present in an amount of 0 to 10% (v/v),
- the inorganic salt is present in an amount of 0.5 to 8 M, and
- the buffer is present in an amount of 0 to 200 mM.
A method for quantitatively detecting the amount of HBsAg in a sample containing HBsAg, comprising the following steps:
- (1) contacting the sample with a first antibody capable of specifically binding to HBsAg in a reagent composition to obtain an immune complex, wherein the reagent composition comprises tris(2-carboxyethyl)phosphine hydrochloride (TCEP), urea, a non-ionic surfactant, an inorganic salt and a buffer, wherein:
- the TCEP is present in an amount of 1 to 100 mM;
- the urea is present in an amount of 0.5 to 8 M;
- the non-ionic surfactant is present in an amount of 0 to 10% (v/v),
- the inorganic salt is present in an amount of 0.5 to 8 M, and
- the buffer is present in an amount of 0 to 200 mM;
- and
- (2) determining the amount of the immune complex obtained in step (1);
- wherein the sample is a blood sample.
Dependencies are inferred from the claim text.