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US 11,543,404 B2Granted
Nerve cell device and method for evaluating neural activity
AssigneeStem Cell & Device Laboratory, Inc.
InventorsKazuhiro Aiba, Norie Tooi, Kosuke Hori, Masaki Sudo
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Abstract
The present invention provides a nerve cell device in which early observation of nerve activity (spikes, bursts, and the like) is made possible and the measured electric strength is increased by cultivating neurons upon a cell scaffold. By using this nerve cell device, imaging of intracellular signaling is also possible.
Claims10
A nerve cell device comprising
- a cell scaffold and neurons,
- wherein the cell scaffold is a fiber sheet formed of a polymeric material, wherein the fiber sheet has an oriented structure in which fibers constituting the fiber sheet are aligned in one direction and when the angle in one direction is set to 0° , 80% or more of fibers are present in a range of ±30°, and
- wherein a distance between fibers is 5 to 50 μm in the oriented structure, and
- wherein a diameter of cross section of a fiber constituting the fiber sheet is 0.1 to 8 μm, and
- wherein a thickness of the fiber sheet is 1 to 40 μm, and
- an opening ratio of the fiber sheet is 10 to 50%, and
- wherein the neurons form a three-dimensional structure on the cell scaffold and/or in the cell scaffold, and neurons are seeded at a density of 1×104 cells/cm2 to 4×106 cells/cm2 against the cell scaffold.
4 more elements
Dependencies are inferred from the claim text.