Isolation and analysis of terpenes
AssigneeOrange Photonics, Inc.
InventorDylan Elmer Wilks
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Abstract
In accordance with embodiments of the present invention, a terpene-rich sample is prepared for terpene analysis using liquid chromatography via an extraction method that takes little time, uses minimal external equipment, and permits direct injection of extracted terpenes into a liquid chromatography instrument for analysis. An embodiment of the invention involves preparing a terpene-containing sample for analysis by liquid chromatography by liquid extraction; heating the liquid extract in a vial that contains a filter medium or solvent; collecting the terpenes in the medium by the vapor pressure forced through the filter from heating; and eluting the collected terpenes into a vial or directly into a chromatography injector.
Claims18
A method of analyzing terpenes in a sample, the method comprising the steps of:
- a. weighing and preparing the sample for extraction;
- b. adding, to a container, the sample and an extraction solvent;
- c. agitating the vial;
- d. heating the sample to force terpenes and the extraction solvent through a collection filter having therein a medium that binds terpenes;
- and
- e. eluting bound terpenes from the medium for analysis,
- wherein the terpenes are eluted from the medium and injected into a chromatography instrument in a single step.
A method of analyzing terpenes in a sample, the method comprising the steps of:
- a. weighing and preparing the sample for extraction;
- b. adding, to a container, the sample and an extraction solvent;
- c. agitating the vial;
- d. heating the sample to force terpenes and the extraction solvent through a collection filter having therein a medium that binds terpenes;
- and
- e. eluting bound terpenes from the medium for analysis,
- wherein the extraction solvent is heated below its boiling temperature but above a volatilization temperature of the terpenes in the sample.
Dependencies are inferred from the claim text.