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US 11,542,511 B2Granted
Recombinant microorganism having simultaneous fermentation ability of at least two sugars and method for producing diol using same
AssigneeGs Caltex Corporation
InventorsJong-Myoung Park, Chelladurai Rathnasingh, Hyo-Hak Song
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Abstract
The present invention relates to a recombinant microorganism which is capable of simultaneously fermenting at least two sugars in a lignocellulosic saccharified liquid, and also capable of generating diol.
Claims7
A recombinant Klebsiella having simultaneous fermentation ability of glucose and xylose and ability to produce 2,3-butanediol, the recombinant Klebsiella is transformed to have a deletion of an ldhA gene having the nucleic acid of SEQ ID NO:
- 1 and a deletion of a pflB gene having the nucleic acid of SEQ ID NO:
- 2;
- and transformed to have a deletion of a crr gene having the nucleic acid of SEQ ID NO:3, and/or a deletion of a ptsG gene having the nucleic acid of SEQ ID NO:
- 4;
- and transformed to overexpress a plasmid comprising a xylA gene having the nucleic acid of SEQ ID NO:
- 5 and a xylE gene having the nucleic acid of SEQ ID NO:
- 6;
- overexpress a plasmid comprising a rpe gene having the nucleic acid of SEQ ID NO:
- 7;
- overexpress a plasmid comprising a rpiA gene having the nucleic acid of SEQ ID NO:
- 8;
- overexpress a plasmid comprising a talE gene having the nucleic acid of SEQ ID NO:
- 9;
- overexpress a plasmid comprising a tktAB gene having the nucleic acid of SEQ ID NO:
- 12;
- overexpress a plasmid comprising a crp(in)01 gene having the nucleic acid of SEQ ID NO:
- 13;
- and/or overexpress a plasmid comprising a crp(in)02 gene having the nucleic acid of SEQ ID NO:
- 14.
16 more elements
Dependencies are inferred from the claim text.