Rice serine hydroxymethyltransferase coded gene OsSHM4 mutants and application thereof
AssigneeNanjing Agricultural University
InventorsFangjie Zhao, Jie Chen, Zhong Tang, Xinyuan Huang
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AssigneeNanjing Agricultural University
InventorsFangjie Zhao, Jie Chen, Zhong Tang, Xinyuan Huang
Estimating the term…
The present invention discloses rice serine hydroxymethyltransferase coded gene OsSHM4 mutants and application thereof. The rice serine hydroxymethyltransferase coded gene mutants are obtained in a manner that T at a 461st position of a CDS sequence region of a wild type rice OsSHM4 gene is mutated to C, so that coded amino acids thereof are mutated from leucine to proline. A CDS sequence of the wild type rice OsSHM4 gene is shown in SEQ ID NO. 1. After mutation of serine hydroxymethyltransferase coded genes OsSHM4, under a field experiment condition, S and Se content of rice shoots is remarkably improved without influencing biomass of rice. After wild type serine hydroxymethyltransferase coded genes OsSHM4 are genetically modified to complement the mutants, S and Se content of shoots of complemented strains is restored to that of wild type rice water planting.
A nucleic acid molecule encoding a rice serine hydroxymethyltransferase mutant having the amino acid sequence set forth as SEQ ID NO:
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