Display substrate, manufacturing method thereof, and display device

A display substrate, comprising:

• a base substrate;
• a pixel defining layer on the base substrate, the pixel defining layer comprising a plurality of openings, wherein, in a direction from a position close to the base substrate to a position away from the base substrate, the pixel defining layer comprises a first pixel defining layer, a conductive layer, and a second pixel defining layer which are stacked, in the pixel defining layer in at least a peripheral region of the display substrate, an orthographic projection of the conductive layer on the base substrate completely falls within an orthographic projection of the second pixel defining layer on the base substrate;
• and
• an electroluminescent unit, located in the openings, the electroluminescent unit comprising a light emitting layer and a transparent electrode on a side of the light emitting layer away from the base substrate,
• wherein the transparent electrode is electrically connected with the conductive layer in the pixel defining layer in at least the peripheral region of the display substrate,
• wherein a cross section of the second pixel defining layer has a trapezoid shape, and a length of a base of the trapezoid shape away from the base substrate is greater than a length of a base of the trapezoid shape close to the base substrate,
• wherein a surface of the conductive layer facing the second pixel defining layer and a surface of the second pixel defining layer facing the conductive layer are the same in shape and size.