Method for digitally correcting an optical image of a sample by means of a microscope, and microscope
AssigneeLeica Microsystems CMS GmbH
InventorsChristian Schumann, Tobias Bauer
Life of this patent
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Abstract
A method is useable for digitally correcting an optical image of a sample by a microscope that has a cover slip covering the sample. The method includes: determining, by the microscope, an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip; ascertaining an imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, the tilt of the cover slip, and/or the thickness of the cover slip; carrying out imaging of the sample by the microscope; and digitally correcting image data captured by the imaging of the sample based on the pupil function.
Claims12
A method for digitally correcting an optical image of a sample by a microscope that has a cover slip covering the sample, the method comprising:
- determining, by the microscope, an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip;
- ascertaining an imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, the tilt of the cover slip, and/or the thickness of the cover slip;
- carrying out imaging of the sample by the microscope;
- and
- digitally correcting image data captured by the imaging of the sample based on the pupil function.
A microscope, comprising:
- a cover slip for covering a sample;
- and
- a device configured to digitally correct an optical image of the sample by:
- determining an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip;
- ascertaining the imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, a tilt of the cover slip, and/or the thickness of the cover slip;
- and
- digitally correcting image data captured by the imaging of the sample based on the pupil function.
Dependencies are inferred from the claim text.