Bladder cancer biomarker proteins

An artificial protein comprising at least 10 signature peptides representing at least 10 proteins selected from the group consisting of

• A1AT_HUMAN (Alpha-1-antitrypsin)
• A2MG_HUMAN (Alpha-2-macroglobulin)
• ALDOA_HUMAN (Fructose-bisphosphate aldolase A)
• DPP4_HUMAN (Dipeptidyl peptidase 4)
• EGF_HUMAN (Pro-epidermal growth factor)
• ITIH2_HUMAN (Inter-alpha-trypsin inhibitor heavy chain H2)
• LG3BP_HUMAN (Galectin-3-binding protein)
• PIP_HUMAN (Prolactin-inducible protein)
• SORL_HUMAN (Sortilin-related receptor)
• TRFE_HUMAN (Serotransferrin)
• VTDB_HUMAN (Vitamin D-binding protein)
• ES8L2_HUMAN (Epidermal growth factor receptor kinase substrate 8-like protein 2)
• HBA_HUMAN (Hemoglobin subunit alpha)
• HBB_HUMAN (Hemoglobin subunit beta)
• HPT_HUMAN (Haptoglobin)
• K1C17_HUMAN (Keratin, type I cytoskeletal 17)
• PRDX4_HUMAN (Peroxiredoxin-4)
• RAI3_HUMAN (Retinoic acid-induced protein 3)
• RAP2A_HUMAN (Ras-related protein Rap-2a) and
• RAP2B_HUMAN (Ras-related protein Rap-2b),
• wherein the signature peptides consist of sequences selected from the group consisting of:
• ITPNLAEFAFSLYR (SEQ ID NO:
• 4, representing A1AT_HUMAN),
• LQHLENELTHDIITK (SEQ ID NO:
• 5, representing A1AT_HUMAN),
• LSITGTYDLK (SEQ ID NO:
• 6, representing A1AT_HUMAN),
• SVLGQLGITK (SEQ ID NO:
• 8, representing A1AT_HUMAN),
• LVHVEEPHTETVR (SEQ ID NO:
• 14, representing A2MG_HUMAN),
• NEDSLVFVQTDK (SEQ ID NO:
• 15, representing A2MG_HUMAN),
• QGIPFFGQVR (SEQ ID NO:
• 16, representing A2MG_HUMAN),
• GILAADESTGSIAK (SEQ ID NO:
• 23, representing ALDOA_HUMAN),
• VLEDNSALDK (SEQ ID NO:
• 103, representing DPP4_HUMAN),
• WEYYDSVYTER (SEQ ID NO:
• 104, representing DPP4_HUMAN),
• IESSSLQGLGR (SEQ ID NO:
• 105, representing EGF_HUMAN),
• IYWVDLER (SEQ ID NO:
• 106, representing EGF_HUMAN),
• LFWIQYNR (SEQ ID NO:
• 107, representing EGF_HUMAN),
• FYNQVSTPLLR (SEQ ID NO:
• 156, representing ITIH2_HUMAN),
• IQPSGGTNINEALLR (SEQ ID NO:
• 157, representing ITIH2_HUMAN),
• TEVNVLPGAK (SEQ ID NO:
• 158, representing ITIH2_HUMAN),
• ELSEALGQIFDSQR (SEQ ID NO:
• 184, representing LG3BP_HUMAN),
• SDLAVPSELALLK (SEQ ID NO:
• 187, representing LG3BP_HUMAN),
• ELGICPDDAAVIPIK (SEQ ID NO:
• 212, representing PIP_HUMAN),
• TFYWDFYTNR (SEQ ID NO:
• 220, representing PIP_HUMAN),
• TVQIAAVVDVIR (SEQ ID NO:
• 221, representing PIP_HUMAN),
• TYLISSIPLQGAFNYK (SEQ ID NO:
• 222, representing PIP_HUMAN),
• YTACLCDDNPK (SEQ ID NO:
• 223, representing PIP_HUMAN),
• AADLLLHSK (SEQ ID NO:
• 266, representing SORL_HUMAN),
• TNVYISSSAGAR (SEQ ID NO:
• 268, representing SORL_HUMAN),
• SASDLTWDNLK (SEQ ID NO:
• 276, representing TRFE_HUMAN),
• THLPEVFLSK (SEQ ID NO:
• 282, representing VTDB_HUMAN),
• VGPQVPLSEPGFR (SEQ ID NO:
• 379, representing ES8L2_HUMAN)
• MFLSFPTTK (SEQ ID NO:
• 393, representing HBA_HUMAN),
• TYFPHFDLSHGSAQVK (SEQ ID NO:
• 394, representing HBA_HUMAN),
• VGAHAGEYGAEALER (SEQ ID NO:
• 395, representing HBA_HUMAN),
• FLASVSTVLTSK (SEQ ID NO:
• 396, representing HBA_HUMAN),
• SAVTALWGK (SEQ ID NO:
• 398, representing HBB_HUMAN),
• VNVDEVGGEALGR (SEQ ID NO:
• 399, representing HBB_HUMAN),
• EFTPPVQAAYQK (SEQ ID NO:
• 400, representing HBB_HUMAN),
• VGYVSGWGR (SEQ ID NO:
• 402, representing HPT_HUMAN),
• VTSIQDWVQK (SEQ ID NO:
• 403, representing HPT_HUMAN),
• VVLHPNYSQVDIGLIK (SEQ ID NO:
• 404, representing HPT_HUMAN)
• DYAEVGR (SEQ ID NO:
• 405, representing HPT_HUMAN),
• VQFELHYQEVK (SEQ ID NO:
• 444, representing ITIH2_HUMAN),
• ALEEANTELEVK (SEQ ID NO:
• 451, representing K1C17_HUMAN),
• TIEELQNK (SEQ ID NO:
• 453, representing K1C17_HUMAN),
• DYGVYLEDSGHTLR (SEQ ID NO:
• 524, representing PRDX4_HUMAN),
• IPLLSDLTHQISK (SEQ ID NO:
• 525, representing PRDX4_HUMAN),
• VSVADHSLHLSK (SEQ ID NO:
• 526, representing PRDX4_HUMAN)
• QITLNDLPVGR (SEQ ID NO:
• 528, representing PRDX4_HUMAN),
• AHAWPSPYK (SEQ ID NO:
• 545, representing RAI3_HUMAN),
• TNVNVFSELSAPR (SEQ ID NO:
• 546, representing RAI3_HUMAN),
• VPVILVGNK (SEQ ID NO:
• 553, representing RAP2A_HUMAN),
• ASVDELFAEIVR (SEQ ID NO:
• 556, representing RAP2B_HUMAN),
• ASNLLLGFDR (SEQ ID NO:
• 575, representing SORL_HUMAN),
• LLGNVLVCVLAHHFGK (SEQ ID NO:
• 917, representing HBB_HUMAN), and
• SVSCPLLSR (SEQ ID NO:
• 919, representing ES8L2_HUMAN), and
• wherein each signature peptide represents a single protein.