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US 11,536,715 B2Granted
Quantitative DNA-based imaging and super-resolution imaging
AssigneePresident and Fellows of Harvard College
InventorsRalf Jungmann, Peng Yin, Mingjie Dai, Maier S. Avendano Amado, Johannes B. Woehrstein
Life of this patent
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Abstract
The present disclosure provides, inter alia, methods and compositions (e.g., conjugates) for imaging, at high spatial resolution, targets of interest.
Claims17
A method of detecting a target in a sample, the method comprising:
- contacting the sample with (a) at least one antibody-DNA conjugate that comprises an antibody linked to a docking strand, and (b) at least one fluorescently-labeled imager strand that is complementary to the docking strand of the at least one antibody-DNA conjugate, wherein the at least one fluorescently-labeled imager strand is capable of transiently binding to the docking strand for about 0.1 to about 10 seconds at room temperature;
- and
- imaging fluorescence blinking produced by the binding of the at least one fluorescently-labeled imager strand to the docking strand of the at least one antibody-DNA conjugate, thereby determining whether the at least one antibody-DNA conjugate binds to the target in the sample.
Dependencies are inferred from the claim text.