Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest

A device for analyzing a fluidic sample, the device comprising:

• (a) a fluid inlet;
• (b) a first sample region in fluid communication with the fluid inlet, wherein the first sample region comprises at least one transducer configured to detect an energy property of the fluidic sample and generate a first sample reading, wherein the first sample reading is indicative of osmolarity of the fluidic sample;
• (c) a second sample region in fluidic communication with the first sample region and shaped to receive a volume of 10 nL to 500 nL of the fluidic sample, the second sample region comprising a detection substrate configured to permit detection of one or more analytes in the volume of the fluidic sample to generate a second sample reading;
• (d) a first valve disposed between the first sample region and the second sample region, wherein the first valve controls fluid flow between the first sample region and the second sample region, wherein at least a portion of the detection substrate is positioned substantially into a first end of the first valve and spaced apart from the first sample region;
• (e) a fluid reservoir disposed within the device and in fluidic communication with the second sample region, the fluid reservoir containing a transfer fluid, which when transferred to the second sample region is capable of hydrating a reagent disposed within the second sample region, washing the detection substrate during operation of the device, or both;
• and
• (f) a second valve disposed between the fluid reservoir and the second sample region.