Method for determining concentration of polyelectrolytes and phosphonates
AssigneeKemira Oyj
InventorsSalla Puupponen, Sari Krapu
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Abstract
The present invention provides a method for determining concentration of polyelectrolyte or phosphonate in a sample comprising polyelectrolyte or phosphonate in low concentrations. The method comprises admixing the sample with a reagent comprising a lanthanid(lll) ion; admixing the sample with silica; allowing the polyelectrolyte or phosphonate in the sample to interact with the reagent comprising the lanthanide(lll) ion and the silica; exciting the sample and detecting a sample signal deriving from the lanthanide(lll) ion by time-resolved fluorescence measurement; and determining the concentration of the polyelectrolyte or phosphonate in the sample by using the detected sample signal.
Claims20
A method for determining concentration of polyelectrolyte or phosphonate in a sample comprising polyelectrolyte or phosphonate, the method comprising:
- optionally diluting and/or purifying the sample;
- admixing the sample with a reagent comprising a lanthanide(lll) ion;
- admixing the sample with silica;
- allowing the polyelectrolyte or phosphonate in the sample to interact with the reagent comprising the lanthanide(lll) ion and the silica;
- exciting the sample at a excitation wavelength and detecting a sample signal deriving from the lanthanide(lll) ion at a signal wavelength by using time-resolved fluorescence measurement;
- and
- determining the concentration of the polyelectrolyte or phosphonate in the sample by using the detected sample signal.
Dependencies are inferred from the claim text.