Methods and systems for analysis of samples containing particles used for gene delivery

A method, comprising:

• introducing, into each capillary from a plurality of capillaries, a sample from a plurality of samples, each sample from the plurality of samples including a different known proportion of Adeno-associated virus (AAV) vectors that include an amount of nucleic acid (NA) content;
• focusing, in each capillary from the plurality of capillaries, at least one analyte from the sample in that capillary using isoelectric focusing;
• labeling each sample from the plurality of samples in each capillary from the plurality of capillaries with a binder configured to bind to at least a portion of a specified NA content;
• detecting a signal associated with the binder from each sample from the plurality of samples;
• and
• determining a relationship between the signal from each sample and the known proportion of AAV vectors that include the specified NA content in that sample.

A method, comprising:

• introducing a sample containing a plurality of viral vectors in a conductive medium into a plurality of capillaries, a first end of each capillary from the plurality of capillaries being ionically coupled to a first running buffer, a second end of each capillary from the plurality of capillaries being ionically coupled to a second running buffer such that a pH gradient is formed along each capillary from the plurality of capillaries;
• separating the plurality of viral vectors in the sample in each capillary by applying a voltage between the first running buffer and the second running buffer;
• incubating the plurality of viral vectors in a first capillary from the plurality of capillaries with a first binder configured to selectively bind to a portion of a nucleic acid (NA) included in a first subset of the viral vectors from the plurality of viral vectors;
• incubating the plurality of viral vectors in a second capillary from the plurality of capillaries with a second binder configured to selectively bind to a portion of a protein included in a second subset of viral vectors from the plurality of viral vectors;
• detecting a first signal associated with the first binder to determine a quantity of the NA included in the first subset of viral vectors to which the first binder is bound in the first capillary;
• and
• detecting a second signal associated with the second binder to determine a quantity of the protein included in the second subset of viral vectors to which the second binder is bound in the second capillary.